239IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN PORCINE PARTHENOTES AT 2-CELL AND BLASTOCYST STAGES USING ANNEALING CONTROL PRIMER TECHNOLOGY
نویسندگان
چکیده
منابع مشابه
Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system.
The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method that involves an...
متن کاملAnnealing control primer system for identification of differentially expressed genes on agarose gels.
We developed GeneFishing technology, an improved method for the identification of differentially expressed genes (DEGs) using our novel annealing control primer (ACP) system. Because of high annealing specificity during PCR using the ACP system, the application of the ACP to DEG discovery generates reproducible, authentic, and long (100 bp to 2 kb) PCR products that are detectable on agarose ge...
متن کاملIdentification of drought induced differentially expressed genes in barley leaves using the annealing control-primer-based GeneFishing technique
In the present study, we have used an annealing-control-primer (ACP)-based differential display reverse transcription-polymerase chain reaction (RT-PCR) method to identify drought-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 12 up-regulated and 3 down-regulated genes were identified and sequenced. Temporal expression patterns of the 15 upor d...
متن کاملIdentification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system.
HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a r...
متن کاملIdentification of maternal mRNAs in porcine parthenotes at the 2-cell stage: a comparison with the blastocyst stage.
Successful embryonic development is dependent on the temporal and stage-specific expression of appropriate genes. Currently, information on specific gene expression during early cleavage-stage embryos before zygotic gene activation (ZGA) is limited. In the present study, we compare gene expression between porcine 2-cell and blastocyst stage parthenotes to identify genes that are specifically or...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2004
ISSN: 1031-3613
DOI: 10.1071/rdv16n1ab239